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Virtual Laboratory

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Virtual Pharmacology
Experiments

Comprehensive curriculum covering B.Pharm and D.Pharm standards. Explore simulations and academic modules.

SECTION A

Introduction & Basic Concepts

Experiment #1

Introduction to Pharmacology, Experimental Pharmacology, its Scope, Objectives, and Foundational Concepts

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Experiment #2

Study of Commonly Used Instruments in Experimental Pharmacology

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Experiment #3

Study of common laboratory animals in experimental pharmacology.

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Experiment #4

Introduction to in-vitro pharmacology and physiological salt solutions

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Experiment #5

Study of dose calculation and stock solution preparation in pharmacological experiments.

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Experiment #6

Preclinical Experiments: Types and Details (In Vivo, In Vitro, Ex Vivo)

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SECTION B

Ethics, Handling & Techniques

Experiment #7

Maintenance of laboratory animals as per CPCSEA guidelines (handling & restraining).

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Experiment #8

Common laboratory techniques: Blood withdrawal; Serum and plasma separation; Anaesthetics and euthanasia for animal studies.

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Experiment #9

Study of different routes of drug administration in small animals (rats/mice).

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SECTION C

Autonomic & Peripheral Pharmacology

Experiment #10

Effect of drugs on rabbit eye (pupil size / intraocular pressure).

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Experiment #11

Effect of drugs on frog’s ciliary motility in frog oesophagus.

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Experiment #12

Effect of agonists and antagonists on guinea pig ileum.

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Experiment #13

Effect of saline purgative on frog intestine.

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Experiment #14

Effect of spasmogens and spasmolytics using rabbit jejunum.

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Experiment #15

Effect of Drugs on Gastrointestinal Motility in Mice/Rats

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SECTION D

Central Nervous System (CNS)

Experiment #16

Effect of skeletal muscle relaxants using Rota-rod apparatus.

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Experiment #17

Effect of drugs on locomotor activity using actophotometer.

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Experiment #18

Study of stereotype behavior of drugs on rat/mice.

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Experiment #19

Study of Anti-Catatonic (Anti-Cataleptic) Activity in Rats/Mice

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Experiment #20

Anticonvulsant Activity by Maximal Electroshock Seizure (MES) or PTZ Method

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Experiment #21

Study of anxiolytic activity of drugs using elevated plus maze method.

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Experiment #22

Taming and hypnosis potentiating effect of chlorpromazine in mice/rat.

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SECTION E

Cardiovascular & Renal Pharmacology

Experiment #23

Study of isolation and perfusion of frog heart.

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Experiment #24

Study of effect of calcium ions and potassium ions on isolated frog heart

A. Study of effect of potassium ions on isolated frog heart
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B. Study of effect of calcium ions on isolated frog heart
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Experiment #25

Study of effect of acetylcholine and adrenaline on isolated frog heart

A. Study of effect of acetylcholine on isolated frog heart
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B. Study of effect of adrenaline on isolated frog heart
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Experiment #26

Effect of drugs on blood pressure and heart rate of dog.

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Experiment #27

Study of diuretic activity of drugs using rats/mice.

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SECTION F

Analgesic & Anti-Inflammatory

Experiment #28

Evaluation of analgesic activity by hot plate method.

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Experiment #29

Evaluation of analgesic activity by tail flick method.

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Experiment #30

Evaluation of anti-inflammatory activity using carrageenan-induced paw edema model.

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Experiment #31

Test for pyrogens (rabbit method).

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SECTION G

Endocrine & Metabolic Pharmacology

Experiment #32

Study of insulin-induced hypoglycaemic effect in rabbit.

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SECTION H

Gastrointestinal & Anti-Ulcer

Experiment #33

Study of anti-ulcer activity using pylorus-ligated (Shay) rat model and NSAIDs-induced ulcer model.

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SECTION I

Anaesthetics & Allergy Models

Experiment #34

Study of different types of Local Anasthetic Techniques

A. Study surface anaesthetic activity by loss of corneal reflex in rabbit
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B. Study infiltration anaesthetic activity by loss of cutaneous sensation in guinea pig wheal
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C. Study conduction anaesthetic activity by loss of reflex withdrawal in frog
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Experiment #35

Evaluation of anti-allergic activity by mast cell stabilization assay.

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Experiment #36

Effect of diphenhydramine in experimentally produced asthma in guinea pig.

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SECTION J

In-Vitro Bioassay Studies

Experiment #37

Introduction to bioassay & assembly setup.

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Experiment #38

Dose-response curve (DRC) of acetylcholine using frog rectus abdominis muscle.

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Experiment #39

Effect of physostigmine on DRC of acetylcholine using frog rectus abdominis muscle & rat ileum.

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Experiment #40

Effect of atropine on DRC of acetylcholine using frog rectus abdominis muscle & rat ileum.

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Experiment #41

Bioassay of histamine using guinea pig ileum by matching method.

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Experiment #42

Bioassay of oxytocin using rat uterine horn by interpolation method.

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Experiment #43

Bioassay of serotonin (5-HT) using rat fundus strip by three-point method.

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Experiment #44

Bioassay of acetylcholine using rat ileum/colon by four-point method.

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Experiment #45

Determination of PA₂ value of prazosin using rat anococcygenus muscle (Schild’s plot method).

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Experiment #46

Determination of PD₂ value using guinea pig ileum.

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SECTION K

Toxicology Studies

Experiment #47

Determination of acute oral toxicity (LD₅₀) of a drug from given data.

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Experiment #48

Determination of acute skin irritation/corrosion of a test substance.

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Experiment #49

Determination of acute eye irritation/corrosion of a test substance.

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SECTION L

Biomechanics & Biostatistics

Experiment #50

Estimation of serum biochemical parameters using semi-autoanalyser.

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Experiment #51

Calculation of pharmacokinetic parameters from given data.

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Experiment #52

Biostatistical methods in experimental pharmacology: Student’s t-test; ANOVA; Chi-square test; Wilcoxon signed-rank test.

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